Abstract: Anatomic and Histologic Investigation of Nasolabial Rejuvenation with Wire Subcision and Adjunctive Filler Injection

Suday, Sptem er 5, 2016 MethodS and MaterialS: Sprague Dawley rats were injected subcutaneously with two HA fillers, VYC20L [20 mg/mL] or HYC-24L+ [24 mg/mL], to create a projecting bolus. Four days post-injection, recombinant human hyaluronidase (HX) or ovine hyaluronidase (VIT) were administered at varying dose levels (5U/0.1mL bolus, 10U/0.1mL bolus, and 30U/0.1mL bolus). 3D images were captured to quantify the loss of projection at six time points over 72 hours. Histology was performed to confirm degradation at 2 weeks post-administration.